Hepatoprotective Effects of Biochanin A Against Acetaminophen-induced Liver Toxicity in Mice

Background: Acetaminophen (APAP), a common analgesic agent, is hepatotoxic in overdose. N-acetylcysteine (NAC), as an APAP antidote, shows anaphylactic reactions and has low efficacy poisoning high doses. Objectives: This study was designed to examine the hepatoprotective effect of biochanin A (BA) mice model acetaminophen-induced hepatotoxicity. Methods: To evaluate APAP-induced oxidative stress, liver tissue level malondialdehyde (MDA) glutathione (GSH) activities serum alanine aminotransferase (ALT), aspartate (AST), alkaline phosphatase (ALP), catalase, superoxide dismutase (SOD) were measured. Histological analysis performed. Results: hepatotoxicity manifested by inflammation, acinar hepatic necrosis, fatty degeneration, well increase levels ALP, ALT, AST, MDA, decrease SOD, catalase (CAT), GSH. Pre-treatment with BA at doses (10 20 mg/kg) reduced MDA raised GSH, CAT levels. Moreover, it normalized structure tissue. Conclusions: The results this indicated that protected from injury. Protection may be due inhibition which reduces inflammation.